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e14 mesc es e14tg2a cell line  (ATCC)


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    ATCC e14 mesc es e14tg2a cell line
    E14 Mesc Es E14tg2a Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 485 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+e14+mescs/ES-E14TG2a/bio_rxiv__64898__2026__04__02__715958-217-0-6
    Average 96 stars, based on 485 article reviews
    e14 mesc es e14tg2a cell line - by Bioz Stars, 2026-09
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    Article Title: Members of an array of zinc-finger proteins specify distinct Hox chromatin boundaries.
    Article Snippet: M2 Magnetic Beads MilliporeSigma #M8823 FLAG peptide MilliporeSigma F3290 Critical commercial assays RNAeasy Plus Mini kit Qiagen Cat. # 74-134 Superscript III Life Technologies 18080093 PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat. # A25742 Dovetail Micro-C kit Cantana Bio Cat. # 21006 Deposited data Raw and analyzed data (i.e. ChIP-seq, RNA-seq, and Micro-C) This paper NCBI GEO: GSE230482 Differentially expressed genes in Maz KO Ortabozkoyun et al.20 See Ortabozkoyun et al.20 Publicly available data (i.e. ChIP-seq, Hi-C) See Table S1 See Table S1. .. Raw western blot images in figures This paper Mendeley Data: https://doi.org/10.17632/ 48xw9hxvpm.1 Experimental models: Cell lines E14 mESCs: ES-E14TG2a ATCC CRL-1821 E14 mESCs – Patz1 KO This paper N/A E14 mESCs – Znf263 KO This paper N/A E14 mESCs – FH-PATZ1 This paper N/A CTCF degron mESCs Nora et al.9 N/A CTCF degron – Maz KO mESCs This paper N/A Human 293FTs Thermo Fisher Scientific R70007 E14 mESCs – FH-ZNF263 This paper N/A (Continued on next page) e1 Molecular Cell 84, 1–17.e1–e6, September 19, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory #000664 Mouse: C57BL/6J Patz1 KO This paper N/A Oligonucleotides Primers for Patz1 KO gRNA, see Table S6 This paper N/A Primers for Patz1 KO genotyping, see Table S6 This paper N/A Primers for Znf263 KO gRNA, see Table S6 This paper N/A Primers for Znf263 KO genotyping, see Table S6 This paper N/A Primers for CbF-PATZ1 cloning, see Table S6 This paper N/A Primers for pPB-CAG-3xFLAG-HAPATZ1-pgk-hph cloning, see Table S6 This paper N/A RT-qPCR primers for Hox genes, see Table S6 This paper N/A Primers for pPB-CAG-FLAG-HAZNF263 cloning, see Table S6 This paper N/A Recombinant DNA pSpCas9(BB)-2A-GFP (PX458) Addgene #48138 pSpCas9(BB)-2A-Puro (PX459) V2.0 Addgene #62988 pPB-CAG-3xFLAG-empty-pgk-hph Addgene #48754 pPB-CAG-3xFLAG-HA-PATZ1-pgk-hph This paper N/A Super PiggyBac Transposase plasmid System Biosciences #PB210PA-1 pPB-CAG-FLAG-HA-ZNF263 This paper N/A PX458_sgRNA_MAZ This paper N/A PX459_sgRNA_PATZ1 This paper N/A PX459_sgRNA_ZNF263 This paper N/A Software and algorithms Bowtie2 (version 2.3.4.1) Langmead et al.64 https://bowtie-bio.sourceforge.net/ bowtie2/index.shtml; RRID: SCR_016368 Samtools (version 1.9) Li et al.65 https://samtools.sourceforge.net; RRID: SCR_002105 DEseq2 (version 1.26.0) Anders and Huber66 and Love et al.67 http://bioconductor.org/packages/release/ bioc/html/DESeq2.html; RRID: SCR_015687 PANTHER Mi et al.68 https://www.pantherdb.org; RRID: SCR_004869 GeneVenn Nagarajan and Pirooznia, usm.edu https://www.bioinformatics.org/gvenn/; RRID: SRC_012117 Venn Diagram Plotter N/A https://pnnl-comp-massspec.github.io/ Venn-Diagram-Plotter MACS2 Zhang et al.69 https://pypi.org/project/MACS2/; RRID: SCR_013291 Easeq Lerdrup et al.70 https://easeq.net Integrative Genomics Viewer (version 2.16.2) Robinson et al.71 https://igv.org; RRID: SCR_011793 ChIPpeakAnno - Bioconductor Zhu et al.72 http://www.bioconductor.org/packages/ release/bioc/html/ChIPpeakAnno.html; RRID: SCR_012828 (Continued on next page) Molecular Cell 84, 1–17.e1–e6, September 19, 2024 e2



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    (A-B) Schematic representation of the co-IP experimental design. Co-immunoprecipitated proteins from 3xHA-mCLUH sample (IP CLUH) and control sample (IP mock) are identified LC MS/MS. (A) HCT116 cells are transduced to express 3xHA-mCLUH protein. (B) <t>mESC</t> are genome-edited to express an endogenous 3xHA-CLUH protein. The mESCs knock-in clone G12 is used (See Figure S1C). (C-D) Tables summarizing the MS protein identification in HCT116 (C) and mESCs (D) . Total number of proteins identified by mascot with a false discovery rate (FDR) below 1% in IP mock and IP CLUH samples. Five proteins with the highest specific spectral counts in the IP CLUH are shown. Biological replicate samples are numbered from #1 to #3. (E-F) Volcano plots showing the global enrichment of proteins in IP CLUH versus the IP mock. The x-axis shows the log2 fold change (FC) and the y-axis shows the −log10 of the FDR (n=3), obtained using SAINTexpress software . Significantly enriched proteins are shown in red and are defined by a fold change greater than two and a FDR < 0.1 (shown as dashed red line). Selected proteins with the highest spectral count (shown in D) are labeled and identified with a green circle.
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    (A-B) Schematic representation of the co-IP experimental design. Co-immunoprecipitated proteins from 3xHA-mCLUH sample (IP CLUH) and control sample (IP mock) are identified LC MS/MS. (A) HCT116 cells are transduced to express 3xHA-mCLUH protein. (B) <t>mESC</t> are genome-edited to express an endogenous 3xHA-CLUH protein. The mESCs knock-in clone G12 is used (See Figure S1C). (C-D) Tables summarizing the MS protein identification in HCT116 (C) and mESCs (D) . Total number of proteins identified by mascot with a false discovery rate (FDR) below 1% in IP mock and IP CLUH samples. Five proteins with the highest specific spectral counts in the IP CLUH are shown. Biological replicate samples are numbered from #1 to #3. (E-F) Volcano plots showing the global enrichment of proteins in IP CLUH versus the IP mock. The x-axis shows the log2 fold change (FC) and the y-axis shows the −log10 of the FDR (n=3), obtained using SAINTexpress software . Significantly enriched proteins are shown in red and are defined by a fold change greater than two and a FDR < 0.1 (shown as dashed red line). Selected proteins with the highest spectral count (shown in D) are labeled and identified with a green circle.
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    (A-B) Schematic representation of the co-IP experimental design. Co-immunoprecipitated proteins from 3xHA-mCLUH sample (IP CLUH) and control sample (IP mock) are identified LC MS/MS. (A) HCT116 cells are transduced to express 3xHA-mCLUH protein. (B) <t>mESC</t> are genome-edited to express an endogenous 3xHA-CLUH protein. The mESCs knock-in clone G12 is used (See Figure S1C). (C-D) Tables summarizing the MS protein identification in HCT116 (C) and mESCs (D) . Total number of proteins identified by mascot with a false discovery rate (FDR) below 1% in IP mock and IP CLUH samples. Five proteins with the highest specific spectral counts in the IP CLUH are shown. Biological replicate samples are numbered from #1 to #3. (E-F) Volcano plots showing the global enrichment of proteins in IP CLUH versus the IP mock. The x-axis shows the log2 fold change (FC) and the y-axis shows the −log10 of the FDR (n=3), obtained using SAINTexpress software . Significantly enriched proteins are shown in red and are defined by a fold change greater than two and a FDR < 0.1 (shown as dashed red line). Selected proteins with the highest spectral count (shown in D) are labeled and identified with a green circle.
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    (A-B) Schematic representation of the co-IP experimental design. Co-immunoprecipitated proteins from 3xHA-mCLUH sample (IP CLUH) and control sample (IP mock) are identified LC MS/MS. (A) HCT116 cells are transduced to express 3xHA-mCLUH protein. (B) mESC are genome-edited to express an endogenous 3xHA-CLUH protein. The mESCs knock-in clone G12 is used (See Figure S1C). (C-D) Tables summarizing the MS protein identification in HCT116 (C) and mESCs (D) . Total number of proteins identified by mascot with a false discovery rate (FDR) below 1% in IP mock and IP CLUH samples. Five proteins with the highest specific spectral counts in the IP CLUH are shown. Biological replicate samples are numbered from #1 to #3. (E-F) Volcano plots showing the global enrichment of proteins in IP CLUH versus the IP mock. The x-axis shows the log2 fold change (FC) and the y-axis shows the −log10 of the FDR (n=3), obtained using SAINTexpress software . Significantly enriched proteins are shown in red and are defined by a fold change greater than two and a FDR < 0.1 (shown as dashed red line). Selected proteins with the highest spectral count (shown in D) are labeled and identified with a green circle.

    Journal: bioRxiv

    Article Title: CLUH interactome reveals an association to SPAG5 and a proximity to the translation of mitochondrial protein

    doi: 10.1101/2021.07.08.451585

    Figure Lengend Snippet: (A-B) Schematic representation of the co-IP experimental design. Co-immunoprecipitated proteins from 3xHA-mCLUH sample (IP CLUH) and control sample (IP mock) are identified LC MS/MS. (A) HCT116 cells are transduced to express 3xHA-mCLUH protein. (B) mESC are genome-edited to express an endogenous 3xHA-CLUH protein. The mESCs knock-in clone G12 is used (See Figure S1C). (C-D) Tables summarizing the MS protein identification in HCT116 (C) and mESCs (D) . Total number of proteins identified by mascot with a false discovery rate (FDR) below 1% in IP mock and IP CLUH samples. Five proteins with the highest specific spectral counts in the IP CLUH are shown. Biological replicate samples are numbered from #1 to #3. (E-F) Volcano plots showing the global enrichment of proteins in IP CLUH versus the IP mock. The x-axis shows the log2 fold change (FC) and the y-axis shows the −log10 of the FDR (n=3), obtained using SAINTexpress software . Significantly enriched proteins are shown in red and are defined by a fold change greater than two and a FDR < 0.1 (shown as dashed red line). Selected proteins with the highest spectral count (shown in D) are labeled and identified with a green circle.

    Article Snippet: E14 mESC line (ATCC ® CRL-1821 TM ) and derived lines were grown in DMEM (D6429, Sigma-Aldrich), containing 15% of FBS (Gibco), 100 U/mL LIF (Millipore), 0.1 mM 2-ß-mercaptoethanol (Gibco) and 1% Pen-strep (Sigma-Aldrich), on 0.2% gelatin-coated plates.

    Techniques: Co-Immunoprecipitation Assay, Immunoprecipitation, Control, Liquid Chromatography with Mass Spectroscopy, Knock-In, Software, Labeling